FIG 3.

Direct comparison between the PRNT, FRNT (at different analysis stringencies), and sVNT. (A) PRNT. Sixty-six samples were assayed between the PRNT₅₀, PRNT₇₅, PRNT₉₀, and sVNT. One sample was discordant between the sVNT and the PRNT₇₅ and PRNT₉₀ (red dot). Two samples were discordant between the PRNT₅₀ and PRNT₇₅ (blue dots). For the PRNT, negative samples with values below 10 were randomly assigned values of 2, 5, or 8 to more easily visualize the number of negative samples. (B) FRNT. Forty-five presumed positive samples were tested between the FRNT₅₀, FRNT₇₅, and FRNT₉₀ and the sVNT. The same six samples were categorized as negative by the sVNT, FRNT₇₅, and FRNT₉₀ (red dots). One sample was discordant between the FRNT₇₅ and sVNT. For the FRNT, all samples with a value of zero were assigned a value of 1 to more easily visualize the negative samples.