. 2021 Mar 19;59(4):e01295-20. doi: 10.1128/JCM.01295-20

TABLE 3.

C_T values of the positive pools of 5 and of corresponding individual samples making up the pool

Pool no.^c	C_T_5p (pooled PCR) for^a:			C_T_5p interpretation	Sample no.^d	C_T_i (individual PCR) for^b:			C_T_i interpretation
Pool no.^c	N gene	ORF1ab	S gene	C_T_5p interpretation	Sample no.^d	N gene	ORF1ab	S gene	C_T_i interpretation
7₅	18.14	16.77	17.81	Positive	2	19.79	17.83	18.38	Positive
7₅	18.14	16.77	17.81	Positive	1, 3–5	U	U	U	Negative
16₅	23.99	21.64	22.72	Positive	2	21.94	20.43	20.88	Positive
16₅	23.99	21.64	22.72	Positive	1, 3–5	U	U	U	Negative
17₅	29.77	26.98	28.03	Positive	2	28.45	26.13	26.39	Positive
17₅	29.77	26.98	28.03	Positive	1, 3–5	U	U	U	Negative

C_T_5p, C_T values of 5-sample pools. C_T values for 3 PCR targets (N gene, ORF1ab, and S gene) are shown.

C_T_i, C_T values of SARS-CoV-2-positive samples when tested individually; U, undetermined C_T values. C_T values for 3 PCR targets (N gene, ORF1ab, and S gene) are shown.

Total number of pools tested, n = 26.

Total number of individual samples tested, n = 130.