Table 4. Effect of acirculatory warm ischemia time in DCD III.
Acirculatory WIT | Acirculatory WIT | p | |||
---|---|---|---|---|---|
≤ 10 min | > 10 min | ||||
n | 68 | 70 | |||
Characteristics of islet cell isolate | |||||
Beta cell purity (%) | 24 | (17–31) | 21 | (15–30) | 0.308 |
Endocrine purity (% DTZ) | 57 | (49–68) | 53 | (46–60) | 0.184 |
Beta cell number (x106) | |||||
after purification | 74 | (37–128) | 54 | (13–94) | 0.036 |
after 1–5 days culture | 39 | (22–67) | 32 | (10–52) | 0.049 |
IEQ (x 103) | |||||
after purification | 110 | (55–156) | 89 | (31–138) | 0.173 |
Insulin content (μg/106 beta cells) | |||||
after purification | 17 | (12–25) | 19 | (11–27) | 0.485 |
after 1–5 days culture | 15 | (11–20) | 11 | (7–17) | 0.015 |
Comparison of beta cell characteristics in islet cell isolates from DCD III-organs with acirculatory WIT duration up to 10 min and higher. Data are presented as median (IQR) unless otherwise specified, statistical significance (Mann-Whitney U) was assumed at p < 0.05. Missing data (n = 3).