Table 5. Effect of cold preservation solution type on beta cell characteristics.
| DCD III | p | DBD | p | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UW | HTK | IGL-1 | UW | HTK | IGL-1 | |||||||||
| n | 41 | 76 | 24 | 351 | 141 | 115 | ||||||||
| Characteristics of islet cell isolate | ||||||||||||||
| Beta cell purity (%) | 21 | (16–33) | 24 | (17–29) | 28 | (17–31) | 0.631 | 24 | (18–32) | 23 | (16–33) | 23 | (16–32) | 0.789 |
| Endocrine purity (% DTZ) | 59 | (50–70) | 55 | (47–62) | 54 | (47–67) | 0.327 | 59 | (47–70) | 52 | (47–67) | 60 | (50–68) | 0.160 |
| Beta cell number (x106) | ||||||||||||||
| after purification | 54 | (29–76) | 65 | (22–125) | 86 | (46–151) | 0.042 | 82 | (49–127) | 81 | (39–121) | 108 | (65–184) | <0.001 |
| after 1–5 days culture | 30 | (14–42) | 35 | (11–63) | 60 | (25–91) | 0.016 | 51 | (30–81) | 47 | (25–74) | 69 | (41–115) | 0.006 |
| IEQ (x 103) | ||||||||||||||
| after purification | 87 | (42–128) | 100 | (34–155) | 124 | (61–173) | 0.229 | 130 | (79–185) | 117 | (72–174) | 146 | (86–228) | 0.013 |
| Insulin content (μg/106 beta cells) | ||||||||||||||
| after purification | 19 | (11–28) | 17 | (10–25) | 18 | (14–25) | 0.634 | 19 | (12–28) | 17 | (12–27) | 18 | (13–25) | 0.603 |
| after 1–5 days culture | 11 | (6–18) | 13 | (10–18) | 14 | (10–24) | 0.210 | 15 | (11–20) | 14 | (9–20) | 14 | (11–19) | 0.743 |
Comparison of beta cell characteristics in islet cell isolates from DCD III and DBD-organs cold-preserved in UW, HTK or IGL-1. Data are presented as median (IQR) unless otherwise specified, statistical significance (Kruskal Wallis) was assumed at p < 0.05. Missing data (n = 2).