FIG 1.

KSHV reactivation by SBHA. (A) Flow cytometry analysis of RFP expression in rKSHV.219-infected Burkitt lymphoma cell lines BJAB.219 and Raji.219. The cells were analyzed by flow cytometry 36 h after the addition of various concentrations of suberoyl bis-hydroxamic acid (SBHA) to the culture medium. (B) Dose-response relationship between the SBHA concentration and RFP positivity in BJAB.219 and Raji.219 cells. (C) Real-time RT-PCR analysis of KSHV-encoded RTA (left) and vIL-6 (right) mRNA expression in three KSHV-positive PEL cell lines treated with each drug for 48 h. The averages from three independent experiments are shown, and error bars indicate the standard deviations. (D) Western blot analysis of KSHV-encoded proteins RTA, vIL-6, and LANA-1. The cells were treated with each drug for 48 h. The arrowhead indicates LANA-1. DMSO, dimethyl sulfoxide; TPA, 12-O-tetradecanoylphorbol-13-acetate; Pano, panobinostat; NaB, sodium butyrate.