FIG 7.

Activation of signaling pathways of p53 and NF-κB by SBHA. (A) Western blot analysis of p53- and NF-κB-associated proteins. BJAB, Jurkat, and MOLT-4 cells were applied as positive controls. Ac-p53, acetyl-p53; p-p53, phospho-p53; p-IκBα, phospho-IκBα. (B) DNA binding activity of NF-κB p65 determined by an ELISA. SBHA augmented p65 DNA binding activity in nuclear extracts of PEL cells. Data represent the averages from three independent experiments, and error bars indicate standard deviations. Student’s t test was performed to estimate statistical significance (*, P < 0.05; ***, P < 0.001; ****, P < 0.0001).