FIG 10.

Cytokine levels at day 7 from supernatants of CD4:CD8:NK coculture (in vitro model). Each graph presents expression levels for one cytokine. The control infection (purple) had only cells without Gal-9 or an antibody against TIM-3 (αTIM3). The addition of Gal-9 (2 nM) alone (green) or with an antibody against TIM-3 at 5 μg/ml and 7.5 μg/ml (orange and blue) was evaluated. Anti-TIM-3 alone at 5 μg/ml and 7.5 μg/ml (red and pink) was also tested. No differences were observed in the levels of all cytokines analyzed before and after the addition of anti-TIM-3.