FIG 5.

Deglycosylation assay with tunicamycin (TNC). Bars show HIV-1 reactivation, expressed as p24 antigen production (n = 4) in a cellular model based on IL-7. Comparison of p24 antigen values between Gal-9 alone and Gal-9 with TNC showed a reduction, although it was not significant. Similar results were observed with the presence of an antibody against TIM-3 (anti-TIM-3). Comparison with the cellular model alone (Control) showed a significant difference in the p24 antigen values in the cases of Gal-9 alone, Gal-9 with TNC, and Gal-9 with TNC and anti-TIM-3 (αTIM3). Statistical significance was calculated using the Mann-Whitney U test. P values of <0.05 were considered statistically significant. *, P < 0.05; NS, not significant.