FIG 1.

Immunofluorescence for amyloid-β42 and the HSV-1 antigen ICP4 in uninfected and HSV-1-infected hiPSC-derived neurons. hiPSC-derived 2D neuronal cultures were infected at MOIs of 1 to 0.3. Neurons were also infected at an MOI of 0.3 in the presence of antivirals 5BVdU and IFN-α. (a to f) Microphotographs depicting immunostaining of Aβ42 and the HSV-1 antigen ICP4 in uninfected neurons (a), neurons infected at an MOI of 0.3 in the presence of 5BVdU and IFN-α (b), and neurons acutely infected at MOIs of 1 to 0.3 (c to f). Scale bar is 25 μm (a and c, right), 10 μm (b; c, left; d; e; and f). Quantification of Aβ42 immunofluorescence intensity using ImageJ (g). The increase in Aβ42 fluorescence intensity in infected neurons compared to uninfected neurons was assessed using Student's t test. Error bars represent standard deviations.