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Characterization of 3D brain organoids. (a) Hematoxylin and eosin staining of 9-week-old organoids. (b to j) Immunostaining of 8-μm-thick sections of organoids with antibodies recognizing FOXG1 (b), SOX2 (c), vimentin (d), TBR2 (e), Tuj/CUX2 (f), TBR1 (g), calbindin (h), CTIP2 (i), and CTIP2/SATB2 (j). Nuclei were counterstained with Hoechst 33342. Scale bars are 100 μm (a), 75 μm (b, d, and j), 50 μm (c and e), and 250 μm (f, g, h, and i).
