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List of primers used for RT‐qPCR
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| Chicken Mx | TTCACGTCAATGTCCCAGCTTTGC | ATTGCTCAGGCGTTTACTTGCTCC |
| Chicken GADPH | ATCAAGAGGGTAGTGAAGGCTGCT | TCAAAGGTGGAGGAATGGCTGTCA |
| Chicken OAS | GCAGAAGAACTTTGTGAAGTGGC | TCGGCTTCAACATCTCCTTGTACC |
| IBDV segment A | AAGGGCAGCTACGTCGATCTAC | TGGCAACTTCGTCTATGAAAGC |
