TABLE 1.
Transfection of the dl-reporter RNAs in cell lines
| Cell line | Transfected RNA | Luciferase activity (RLU) (mean ± SEM) |
RTA (FLuc/RLuc) | Normalized RTA | |
|---|---|---|---|---|---|
| RLuc | FLuc | ||||
| Vero E6 | dl HCV IRES | 8.60 × 106 ± 247,909 | 4.50 × 104 ± 6,676 | 5.14 × 10−3 | 7.5 |
| dl HCV G266A/G268U | 6.70 × 106 ± 38,358 | 1.753 × 103 ± 204 | 2.89 × 10−4 | 0.4 | |
| dl 5′UTR DENV | 5.89 × 106 ± 381,428 | 4.10 × 103 ± 928 | 6.84 × 10−4 | 1 | |
| HEK 293T | dl HCV IRES | 1.63 × 107 ± 231,008 | 4.79 × 105 ± 77,190 | 2.93 × 10−2 | 12 |
| dl HCV G266A/G268U | 1.27 × 107 ± 342,424 | 1.17 × 104 ± 2,498 | 9.12 × 10−4 | 0.4 | |
| dl 5′UTR DENV | 1.31 × 107 ± 159,300 | 3.19 × 104 ± 4,958 | 2.44 × 10−3 | 1 | |
| BHK-21 | dl HCV IRES | 1.68 × 107 ± 384,921 | 2.54 × 105 ± 20,248 | 1.5 × 10−2 | 13 |
| dl HCV G266A/G268U | 1.40 × 107 ± 397,720 | 4.65 × 103 ± 128 | 3.32 × 10−4 | 0.3 | |
| dl 5′UTR DENV | 1.42 × 107 ± 298,458 | 1.62 × 104 ± 1,078 | 1.14 × 10−3 | 1 | |
| A549 | dl HCV IRES | 1.46 × 106 ± 44,044 | 1.76 × 104 ± 1,360 | 1.21 × 10−2 | 11 |
| dl HCV G266A/G268U | 9.32 × 105 ± 71,568 | 5.17 × 102 ± 50 | 5.55 × 10−4 | 0.5 | |
| dl 5′UTR DENV | 1.07 × 106 ± 47,434 | 1.2 × 103 ± 47 | 1.12 × 10−3 | 1 | |
| Jurkat | dl HCV IRES | 1.44 × 105 ± 11,224 | 1.19 × 103 ± 135 | 8.22 × 10−3 | 5 |
| dl HCV G266A/G268U | 8.6 × 104 ± 13,317 | 1.65 × 102 ± 12 | 1.97 × 10−3 | 1.2 | |
| dl 5′UTR DENV | 1.45 × 105 ± 12,437 | 2.39 × 102 ± 17 | 1.65 × 10−3 | 1 | |
| Huh-7 | dl HCV IRES | 1.07 × 107 ± 938,542 | 7.87 × 104 ± 8,174 | 7.5 × 10−3 | 10 |
| dl HCV G266A/G268U | 8.10 × 106 ± 831,655 | 2.43 × 103 ± 212 | 3.11 × 10−4 | 0.4 | |
| dl 5′UTR DENV | 8.82 × 106 ± 960,521 | 6.58 × 103 ± 696 | 7.66 × 10−4 | 1 | |
| HeLa | dl HCV IRES | 6 × 106 ± 95,609 | 2.42 × 105 ± 12,578 | 4.04 × 10−2 | 15 |
| dl HCV G266A/G268U | 4.11 × 106 ± 143,745 | 5.02 × 103 ± 202 | 1.22 × 10−3 | 0.4 | |
| dl 5′UTR DENV | 4.4 × 106 ± 113,433 | 1.2 × 104 ± 667 | 2.73 × 10−3 | 1 | |
| MDCK | dl HCV IRES | 1.52 × 106 ± 71,888 | 4.84 × 103 ± 1,035 | 3.14 × 10−3 | 6 |
| dl HCV G266A/G268U | 1.16 × 106 ± 52,019 | 6.29 × 102 ± 139 | 5.36 × 10−4 | 1 | |
| dl 5′UTR DENV | 9.84 × 105 ± 54,237 | 5.08 × 102 ± 31 | 5.22 × 10−4 | 1 | |
| C6/36 | dl HCV IRES | 4.53 × 106 ± 211,645 | 9.94 × 102 ± 157 | 2.23 × 10−4 | 0.9 |
| dl HCV G266A/G268U | 2.83 × 106 ± 87,714 | 5.75 × 102 ± 35 | 2.04 × 10−4 | 0.8 | |
| dl 5′UTR DENV | 4.58 × 106 ± 131,534 | 1.14 × 103 ± 122 | 2.5 × 10−4 | 1 | |