TABLE 2.
Transfection of the dl-reporter plasmids in cell lines
Cell line | Transfected DNA | Luciferase activity (RLU) (mean ± SEM) |
RTA (FLuc/RLuc) | Normalized RTA | |
---|---|---|---|---|---|
RLuc | FLuc | ||||
BHK-21 | dl ΔEMCV | 2.070 × 107 ± 264,626 | 0.3 × 105 ± 11,633 | 1.58 × 10−2 | 0.13 |
dl 5′UTR DENV | 1.5 × 107 ± 398,903 | 1.8 × 105 ± 45,206 | 1.19 × 10−1 | 1 | |
Vero E6 | dl ΔEMCV | 5.2 × 106 ± 1,347,204 | 0.02 × 105 ± 1,568 | 4.78 × 10−4 | 0.06 |
dl 5′UTR DENV | 5.3 × 106 ± 1,449,812 | 0.44 × 105 ± 37,428 | 8.24 × 10−3 | 1 | |
HEK 293T | dl ΔEMCV | 5.7 × 106 ± 996,239 | 0.018 × 106 ± 4,222 | 3.16 × 10−3 | 0.96 |
dl 5′UTR DENV | 2.88 × 106 ± 930,362 | 0.09 × 106 ± 3,569 | 3.28 × 10−3 | 1 |