FIG 4.

Neutralization assay of recombinant MAbs using HBV/NL derived from genotype C. MAbs targeting epitopes I (A), II (B), and III (C) were incubated with HBV/NL (20 GEq/cell) for 1 h and then infected into G2/NT-18 cells for 16 h. The luciferase activity of cells was determined at 7 days postinfection and is expressed relative to activity without antibodies. The concentration of antibody that results in 50% neutralization of HBV/NL infection (IC₅₀) was calculated and is shown. Data are averages of quadruplicate values, with error bars showing standard deviations.