TABLE 12.
Oligonucleotide primers used in this study for cloning of phage depolymerases
| Primer | Sequence (5′–3′)a | Restriction site for: |
|---|---|---|
| APK2_dep_F | ataGGATCCgaagaggctgctcagcaaac | BamHI |
| APK2_dep_R | ataAAGCTTaaaatccagataccacagtaa | HindIII |
| APK32_dep_F | ataGGATCCcaagcacaagaggctagcg | BamHI |
| APK32_dep_R | ataCTCGAGttaatctggattaaaaataatatcta | XhoI |
| APK37_dep_F | ataGGATCCgaagcaagtcaagctgctca | BamHI |
| APK37_dep_R | ataAAGCTTaggataatacatggctaatgt | HindIII |
| APK44_dep_F | ataGGATCCgaagctagtgaagctgctca | BamHI |
| APK44_dep_R | ataAAGCTTatttgaaacgatacttaaatcgt | HindIII |
| APK48_dep_F | ataGGATCCgaggaagctgcacaggttac | BamHI |
| APK48_dep_R | ataCTCGAGttaatttactactacactacctag | XhoI |
| APK87_dep_F | ataGGATCCgaagatgcttctgatgcactt | BamHI |
| APK87_dep_R | ataAAGCTTaaataagtttaataagccctcg | HindIII |
| APK89_dep_F | ataGGATCCgaagctagtgaagctgctca | BamHI |
| APK89_dep_R | ataAAGCTTatttaatgtatcgagcgtcac | HindIII |
| APK116_dep_F | ataGGATCCaacgcagcagaagtagctg | BamHI |
| APK116_dep_R | ataAAGCTTagtctttaagatttatgtcgt | HindIII |
a
Uppercase letters indicate restriction endonuclease recognition sites.