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. 2021 Feb 10;95(5):e01714-20. doi: 10.1128/JVI.01714-20

TABLE 7.

1H and 13C NMR chemical shifts of the O-deacetylated LUH5547 (K87) CPS (DPS) and oligosaccharide 5 derived by depolymerization of the CPS with phage depolymerase APK87_gp48a

Residue 1H and 13C chemical shifts (δ, ppm)
H-1
C-1		 H-2
C-2		 H-3
C-3		 H-4
C-4		 H-5
C-5		 H-6a,6b
C-6
DPS (at 50°C)b
 →3)-β-d-GlcpNAc-(1→ A 4.75 3.85 3.66 3.53 3.49 3.78, 3.95
103.2 56.8 82.8 69.7 77.0 62.0
 →3)-α-l-6dTalp-(1→ B 4.97 3.74 3.87 3.88 4.28 1.23
103.2 71.1 72.0 70.3 68.8 16.7
 →2)-α-l-Rhap-(1→ C 5.08 4.01 3.95 3.48 3.75 1.30
97.7 79.8 71.1 73.5 70.5 18.0
 →2,3)-α-l-Rhap-(1→ D 5.16 4.40 3.92 3.56 3.75 1.25
102.4 79.3 81.0 72.0 70.5 17.8f
 →4)-β-d-GlcpA-(1→ E 4.78 3.39 3.77 3.81 3.95
104.8 74.5 77.2 78.3 76.1 174.1
 →2)-α-d-Glcp-(1→ F 5.42 3.55 3.72 3.68 3.54 3.69, 3.77
100.0 78.2 72.8 72.4 73.0 61.1
 α-l-Rhap-(1→ G 4.86 3.98 3.76 3.44 4.00 1.25
102.0 71.7 71.5 73.3 70.3 17.9f
Oligosaccharide 5 (at 40°C)c
 →3)-α-d-GlcpNAc Aα 5.15 4.03 3.82 3.55 3.89 3.80, 3.86
92.4 54.9 80.7 69.8 73.1 62.0
 →3)-β-d-GlcpNAc-(1→ Aβ 4.75 3.79 3.63 3.52 3.49 3.79, 3.92
95.7 57.8 83.1 69.8 77.3 62.1
 →3)-α-l-6dTalp-(1→ B 4.98d 3.73 3.88 3.92 4.30 1.24
103.2e 71.3 72.1 70.3 68.8 16.8
 →2)-α-l-Rhap-(1→ C 5.11 4.04 3.98 3.49 3.78 1.32
97.8 80.3 71.1 73.4g 70.6h 18.1
 →2)-α-l-Rhap-(1→ D 5.27 4.16 3.90 3.50 3.78 1.27
102.4 81.4 71.2 73.6g 70.3h 17.8i
 →4)-β-d-GlcpA-(1→ E 4.62 3.44 3.77 3.80 3.78
105.5 74.7 77.4 78.0 78.3 n.f.
 →2)-α-d-Glcp-(1→ F 5.43 3.55 3.74 3.79 3.54 3.70, 3.81
99.7 78.2 72.9 72.2 73.2 61.2
 α-l-Rhap-(1→ G 4.87 4.00 3.79 3.46 4.03 1.27
102.1 71.8 71.5 73.3 70.3 18.0i
a

1H NMR chemical shifts are italicized. n.f., not found.

b

Chemical shifts for the N-acetyl groups are δH 2.02, δC 23.5 (Me), and 175.5 (CO).

c

Chemical shifts for the N-acetyl groups are δH 2.06, δC 23.3 (Aα), 23.5 (Aβ) (both Me), and 176.3 (CO).

d

When linked to Aα; δ 4.96 when linked to Aβ.

e

When linked to Aα; δ 103.3 when linked to Aβ.

f

Assignment could be interchanged.

g

Assignment could be interchanged.

h

Assignment could be interchanged.

i

Assignment could be interchanged.