TABLE 8.

¹H and ¹³C NMR chemical shifts of the K32 CPS of A. baumannii LUH5549 and oligosaccharide 6 derived by depolymerization of the CPS with phage depolymerase APK32_gp46^a

Residue	1H and 13C chemical shifts (δ, ppm)
	H-1 C-1	H-2 C-2	H-3 C-3	H-4 C-4	H-5 C-5	H-6a, 6b C-6
CPS (at 65°C) (19)
→3)-β-d-GalpNAc-(1→ A	4.54 104.1	3.83 52.5	3.89 81.3	4.06 69.2	3.59 75.9	3.74, 3.82 62.6
→4)-β-d-GlcpA-(1→ B	4.58 105.3	3.40 73.5	3.64 75.2	3.84 81.2	4.01 74.8	172.1
→3,4)-β-d-GalpNAc-(1→ C	4.53 102.9	4.06 52.8	3.84 79.0	4.22 74.9	3.73 77.0	3.64, 3.68 61.6
→6)-α-d-GlcpNAc-(1→ D	4.86 98.3	3.90 55.3	3.87 71.9	3.71 70.9	4.34 72.0	4.06, 4.32 69.3
β-d-Glcp-(1→ E	4.47 104.0	3.35 74.5	3.49 77.2	3.40 71.2	3.43 77.2	3.72, 3.91 62.4
Oligosaccharide 6 (at 30°C)
→3)-α-d-GalpNAc Aα	5.22 92.4	4.29 50.2	4.01 78.6	4.20 69.8	4.13 71.5	3.74, 3.74 62.5
→3)-β-d-GalpNAc Aβ	4.69 96.4	3.99 53.6	3.82 81.5	4.13 69.1	3.69 76.2	3.74, 3.76 62.3
→4)-β-d-GlcpA-(1→ B(α)b	4.57 105.3	3.38 73.9	3.59 75.1	3.78 81.2	3.69 77.8	175.5
→4)-β-d-GlcpA-(1→ B(β)c	4.51 105.5	3.38 73.8	3.59 75.0	3.78 81.2	3.69 77.8	175.5
→4)-β-d-GalpNAc-(1→ C	4.55 102.6	3.97 53.3	3.79 71.7	3.98 77.5	3.74 76.7	3.74, 3.74 61.5
→6)-α-d-GlcpNAc-(1→ D	4.90 99.5	3.95 55.2	3.87 71.7	3.64 70.8	4.31 72.3	3.91, 4.07 69.2
β-d-Glcp-(1→ E	4.48 103.9	3.32 74.4	3.50 76.9	3.40 70.9	3.44 77.1	3.73, 3.91 62.0

^a1H NMR chemical shifts are italicized. Chemical shifts for the N-acetyl groups are δ_H 1.96–2.09, δ_C 23.1–23.8 (Me), and 175.1–176.3 (CO).

^bLinked to Aα.

^cLinked to Aβ.