FIG 5.

Three types of reactivity in mice immunized with the mixture of 10 peptides. Fifteen mice were immunized once with the mixture of 10 peptides, including pp1a-38, -84, -641, -1675, -2884, -3467, -3583, -3662, -3710, and -3732, in liposomes with CpG. After 1 week, spleen cells were stimulated with or without each of the 10 peptides, and intracellular IFN-γ in CD8⁺ T cells was stained. Based on the reactivity pattern to the 10 peptides, 15 mice were divided into three types, A, B, and C. Each graph represents reactivity of an individual mouse. The y axis indicates the relative percentage of IFN-γ⁺ cells in CD8⁺ T cells, which was calculated by subtracting the % of IFN-γ⁺ cells in CD8⁺ T cells without a peptide from the % of IFN-γ⁺ cells in CD8⁺ T cells with a relevant peptide. Statistical analyses of the relative % values to 10 peptides in each type were performed by one-way ANOVA followed by post hoc tests: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.