Table 3. Procoagulant and neutralization activity of Daboia russelii venoms with VPAV.

Venom origin	Minimum coagulation dose (MCD) (µg/mL)	Challenge dose (2 MCD) (µg/mL)	Effective dose, ED (µg/µL)
Daboia russelii (Sri Lanka)	0.0115 ± 0.001	0.023	0.94 (0.87-1.03)
Daboia russelii (India)	0.0132 ± 0.001	0.026	1.1 (0.97-1.26)

MCD was defined as the amount of venom that caused clotting in 1 mL of citrated human plasma in 3 min. Effective dose (ED): the venom amount (µg) neutralized per (µL) of antivenom when the plasma coagulation time at 2 MCD was prolonged three times that of control (without antivenom). Results are presented as mean of triplicate ± S.E.M.