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. 2021 Apr 29;14:2879–2892. doi: 10.2147/OTT.S299234

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© 2021 Chen et al.

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MiR-140-3p directly targeted BCL2 in GC cell lines.

Notes: (A) A putative miR-140-3p binding site in the 3ʹ-UTR of the BCL2 mRNA transcript. Mutations were generated in the complementary domain that directly binds to the seed region of miR-140-3p. (B) A luciferase reporter assay was performed with BGC-823 cells to detect the relative luciferase activities of WT and MUT BCL2 reporters. (C) Biotin-coupled miRNA pull-down assays determined that BCL2 was effectively enriched by miR-140-3p. (D and E) BCL2 mRNA levels were studied by qRT-PCR. (F) BCL2 was negatively regulated by miR-140-3p. **P<0.01; ***P<0.001.

Abbreviations: BCL2-wt, wild-type BCL2 reporter; BCL2-mut, mutant BCL2 reporter; NC, negative control.