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. 2021 Apr 29;14:2879–2892. doi: 10.2147/OTT.S299234

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© 2021 Chen et al.

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BECN1 interfered with autophagy regulated by miR-140-3p in GC cell lines.

Notes: (A) BECN1 was negatively regulated by BCL2. (B) miR-140-3p affected the expression of BCL2. (C) Three siRNAs for knocking down BECN1 were designed and Western blot was used to detect BECN1 expression. (D) Transfection efficiency was determined by Western blot analysis. (E) Immunofluorescence staining for LC3 was performed with GC cells; scale bar = 100 μm. *P<0.05; **P<0.01; ***P<0.001.

Abbreviations: si-BCL2, siRNA targeting BCL2; pc-BECN1, BECN1-expressing vectors; NC, negative control.