TABLE 4.

Oligonucleotides used as PCR primers for cyp51A and cyp51B knockout screening

Primer	Sequence (5′–3′)	Species
Ancyp51A Fw scr	GAACGATGCAGCTCAGTGCCA	A. niger
Ancyp51A Rev scr	ACACGAGGGATCTCACGTGC	A. niger
Ancyp51B Fw scr	CCTGCTACTAGGAGAGCCGC	A. niger
Ancyp51B Rev scr	GCCTGTATGGGACAGTCGCT	A. niger
Atcyp51A Fw scr	CCGATGTAGCTCAGTGCCAGG	A. tubingensis
Atcyp51A Rev scr	ACTGGGCAGCGGTCATATCTC	A. tubingensis
Atcyp51B Fw scr	GTGCACTTTCCTGCGCTGTTG	A. tubingensis
Atcyp51B Rev scr	CCGGCCATTAAAGCCCCAAG	A. tubingensis
Hyg Fw intern	CGATTCCTTGCGGTCCGAAT	All
Hyg Rev intern	ATCGGCACTTTGCATCGGC	All