TABLE 2.

IC₅₀, FICI₅₀, and ΣFICI₅₀ of the combination of itraconazole, ezetimibe, and miltefosine against L. infantum intracellular amastigotes^a

ITR/EZ/MT ratio	Mean IC50 (μM) (SEM)			FICI
	ITR	EZ	MT	ITR	EZ	MT	ΣFICI	${}_{\overline{x}}\Sigma \text{FICI}$
5:0:0	0.89 (0.73–1.08)
4:1:1	0.23 (0.20–0.25)	0.57 (0.52–0.63)	0.038 (0.034–0.04)	0.25	0.049	0.060	0.36
3:2:2	0.07 (0.05–0.11)	0.51 (0.33–0.77)	0.034 (0.022–0.05)	0.078	0.044	0.053	0.17
2:3:3	0.32 (0.24–0.41)	4.81 (3.71–6.24)	0.37 (0.28–0.48)	0.35	0.42	0.58	1.35	0.81
1:4:4	0.029 (0.023–0.037)	1.17 (0.92–1.50)	0.07 (0.06–0.1)	0.032	0.1	0.11	0.24
0:5:0		11.46 (10.1–12.9)
4:1:4	0.83 (0.74–0.93)	2.08 (1.87–2.32)	0.55 (0.49–0.62)	0.93	0.18	0.87	1.98
3:2:3	0.18 (0.15–0.22)	1.25 (1.03–1.50)	0.12 (0.10–0.15)	0.20	0.11	0.19	0.5
2:3:2	0.46 (0.37–0.57)	6.93 (5.60–8.57)	0.3 (0.24–0.38)	0.51	0.60	0.47	1.58
1:4:1	0.062 (0.05–0.078)	2.51 (2.0–3.15)	0.04 (0.03–0.05)	0.069	0.22	0.063	0.35
0:0:5			0.63 (0.37–1.08)

^aIC₅₀, concentration that inhibits 50% of parasite growth (followed by the standard error of the mean [SEM]). According to published guidelines, a fractional inhibitory concentration index (FICI) of 0.5 to 4.0 is indicative of an additive effect.