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EtBr accumulation of all AcrAB mutants. (a) Accumulation rates of acrAB knockout, AcrA WT-AcrB WT, and AcrA WT-AcrB P319L strains. (b) Accumulation rates of AcrA mutants in an AcrB WT background. (c) Accumulation rates of AcrA mutants in an AcrB P319L background. AU, arbitrary units.