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. 2021 Apr 19;65(5):e01589-20. doi: 10.1128/AAC.01589-20

TABLE 3.

MIC values for different AcrB and AcrA mutants against different substratesa

Strain MIC (μg/ml)
 % bile salts
LMMDs
 HMMDs
 PACs
CIP NAL ENR NOR MIN TET ERY NOV EB R6G
AcrB WT-AcrA WT 0.015 8 0.03 0.06 2 2 256 128 512 >1,024 2.5
AcrB WT-AcrA V367G 0.03 4 0.06 0.12 2 4 256 64 256 256 0.625
AcrB WT-AcrA V367S 0.03 8 0.06 0.12 2 4 128 64 256 256 0.625
AcrB WT-AcrA R368A 0.03 4 0.015 0.12 4 2 256 128 512 >1,024 2.5
AcrB WT-AcrA R368E 0.015 8 0.03 0.06 2 2 256 128 512 >1,024 1.25
AcrB WT-AcrA R368Q 0.015 4 0.03 0.06 2 2 256 128 512 >1,024 1.25
AcrB P319L-AcrA WT 0.03 16 0.12 0.25 4 4 512 128 512 >1,024 2.5
AcrB P319L-AcrA V367G 0.06 16 0.12 0.5 8 4 512 256 512 >1,024 2.5
AcrB P319L-AcrA V367S 0.06 16 0.25 0.5 8 8 512 512 512 >1,024 5
AcrB P319L-AcrA R368A 0.06 16 0.12 0.25 4 2 512 256 512 >1,024 2.5
AcrB P319L-AcrA R368E 0.03 8 0.06 0.12 2 2 256 128 256 >1,024 1.25
AcrB P319L-AcrA R368Q 0.03 8 0.06 0.06 2 2 256 128 256 1,024 1.25
a

MIC values for the substrates nalidixic acid (NAL), ciprofloxacin (CIP), enrofloxacin (ENR), norfloxacin (NOR), erythromycin (ERY), novobiocin (NOV), minocycline (MIN), tetracycline (TET), ethidium bromide (EtBr), and rhodamine 6G (R6G) are shown. LMMDs, low-molecular-mass drugs; HMMDs, high-molecular-mass drugs; PACs, planar aromatic cations. The bold text indicates that the MIC value is different from that of the AcrB WT-AcrA WT strain.