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. 2021 Apr 19;65(5):e02621-20. doi: 10.1128/AAC.02621-20

FIG 1.

FIG 1

Expression of mecA (A) and blaZ (B) in the presence and absence of NaHCO3. qRT-PCR was performed using RNA extracted from stationary-phase cells in the presence of 0.5× MIC of oxacillin. Oxacillin concentrations used are the following: MRSA 11/11, without NaHCO3 (w/o), 16 μg/ml; with NaHCO3 (+ NaHCO3), 0.25 μg/ml; MW2, w/o, 32 μg/ml; + NaHCO3, 1 μg/ml; PB 043-043, w/o, 8 μg/ml; + NaHCO3, 0.5 μg/ml; C24, w/o, 16 μg/ml; + NaHCO3, 0.5 μg/ml; COL, w/o, 256 μg/ml; + NaHCO3, 256 μg/ml; BMC1001, w/o, 128 μg/ml; + NaHCO3, 128 μg/ml; PB 027-133, w/o, 128 μg/ml; + NaHCO3, 128 μg/ml; PB 017-037, w/o, 16 μg/ml; + NaHCO3, 16 μg/ml. Black bars indicate growth in CA-MHB 100 mM Tris, white bars indicate growth in CA-MHB 100 mM Tris plus 44 mM NaHCO3, and error bars indicate the CT standard deviations. No blaZ expression was detected in COL, because the strain lacks the blaZ gene. Student’s t test was used for statistical analyses. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. mecA gene expression data for strains marked with a superscript A were previously published (11).