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. 2021 Apr 19;65(5):e02621-20. doi: 10.1128/AAC.02621-20

FIG 3.

FIG 3

Expression of genes related to PBP2a maturation and function in the presence and absence of NaHCO3. (A) vraSR. (B) prsA. (C) pbp4. qRT-PCR was performed using RNA extracted from log-phase cells (OD600, 0.4) in the presence of 0.5× MIC oxacillin for vraSR and prsA or on RNA extracted from stationary-phase cells (pbp4). Black bars indicate growth in CA-MHB with 100 mM Tris, white bars indicate growth in CA-MHB with 100 mM Tris plus 44 mM NaHCO3 for all graphs, and error bars indicate the CT standard deviations. Student’s t test was used for statistical analyses. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.