TABLE 1.

MICs of NOSO-502, CST, and comparators against selected mutants coresistant to NOSO-502 and CST

K. pneumoniae strain	Mutant	NOSO-502 MIC-fold selection	CrrB substitutionb	MIC (μg/ml)a
				NOS	CST	IPM	GEN	TIG
ATCC BAA-2146 WT	NA	NA	NA	1	1	128	>256	32
ATCC BAA-2146	AT_P151S_1	4×	P151S	128	>256	128	>256	64
ATCC BAA-2146	AT_P151S_2	4×	P151S	256	>256	128	>256	64
ATCC BAA-2146	AT_N141I_1	4×	N141I	128	>256	128	>256	64
ATCC BAA-2146	AT_P151S_3	4×	P151S	128	>256	128	>256	64
ATCC BAA-2146	AT_P151S_4	4×	P151S	256	>256	128	>256	64
ATCC BAA-2146	AT_P151S_5	4×	P151S	128	>256	128	>256	64
ATCC BAA-2146	AT_P151S_6	4×	P151S	256	>256	256	>256	32
ATCC BAA-2146	AT_N141I_2	4×	N141I	128	>256	128	>256	32
ATCC BAA-2146	AT_P151S_7	4×	P151S	128	>256	128	>256	64
ATCC BAA-2146	AT_P151S_8	4×	P151S	128	>256	128	>256	64
ATCC BAA-2146	AT_P151S_9	4×	P151S	256	>256	256	>256	64
ATCC BAA-2146	AT_P151S_10	4×	P151S	256	>256	256	>256	64
ATCC BAA-2146	AT_P151S_11	4×	P151S	256	>256	256	>256	64
ATCC BAA-2146	AT_P151S_13	4×	P151S	>256	>256	128	>256	32
ATCC BAA-2146	AT_F303Y_1	4×	F303Y	256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_14	4×	P151S	256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_15	4×	P151S	>256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_16	4×	P151S	256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_17	4×	P151S	256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_18	4×	P151S	256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_19	10×	P151S	256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_20	10×	P151S	256	>256	128	>256	32
ATCC BAA-2146	AT_ F303Y_2	10×	F303Y	256	>256	256	>256	32
ATCC BAA-2146	AT_P151S_21	10×	P151S	256	>256	128	>256	32
ATCC BAA-2146	AT_P151S_22	10×	P151S	>256	>256	256	>256	32
ATCC BAA-2146	AT_ F303Y_3	10×	F303Y	256	>256	128	>256	32
NCTC 13442 WT	NA	NA	NA	1	1	8	≤0.5	2
NCTC 13442	NC_P151L	4×	P151L	256	>256	4	≤0.5	8
NCTC 13442	NC_W140R	4×	W140R	128	>256	16	≤0.5	4
NCTC 13442	NC_P151T	4×	P151T	256	>256	8	≤0.5	8
NCTC 13442	NC_P151A	4×	P151A	256	>256	8	≤0.5	4
NCTC 13442	NC_F33Y	4×	F33Y	64	>256	4	≤0.5	4
NCTC 13442	NC_S8N	4×	S8N	64	>256	8	≤0.5	1
NCTC 13442	NC_Y34N	10×	Y34N	16	>256	8	≤0.5	2

^aNOS, NOSO-502; CST, colistin; IPM, imipenem; GEN, gentamicin; TGC, tigecycline; NA, not applicable.

^bAmino acid positions where mutations in CrrB have been detected.