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. Author manuscript; available in PMC: 2021 May 3.
Published in final edited form as: Neurobiol Dis. 2018 Mar 6;114:120–128. doi: 10.1016/j.nbd.2018.02.014

Fig. 7. Plasma NP1 and NP1 fragments are enriched in exosomes containing fractions isolated from pooled plasma of E4FAD mice.

Fig. 7.

Plasma extracellular vesicles were isolated from pooled plasma of human and E4FAD mice via ultracentrifugation. Western blot analysis revealed that NP1 species were enriched in E4FAD plasma exosome enriched fractions. Western blot results demonstrated levels of NP1 and NP1 fragments in plasma before ultracentrifugation (pla, whole plasma) were reduced or depleted in the supernatant (sup) after pelleting of exosomes (Exo) using ultracentrifugation in two independent pooled plasma groups of E4FAD mice (n = 4/per group). NP1 and its fragments were enriched in the pellet extracellular vesicle fractions, which contain the exosome marker ALIX.