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. Author manuscript; available in PMC: 2021 May 3.
Published in final edited form as: Neurobiol Dis. 2019 Apr 2;127:432–448. doi: 10.1016/j.nbd.2019.02.015

Fig. 4.

Fig. 4

Curcumin dose effects on TREM2 and CD33 protein in activated human THP-1 monocyte cells in vitro. Treatment of Thp-1 cells with high glucose (HG) models NFκB-regulated inflammation. Cells were treated for 1 h with 0.1 μM or 1.5 μM curcumin, then treated with HG (or mannitol control, (CNTR)) for 24 or 72 h, then cells were harvested and proteins quantified on Western blot; TREM2 and CD33 protein levels were normalized to GAPDH protein levels within same sample. (A) Western blot of TREM2 and GAPDH. (B) Levels of TREM2 normalized to GAPDH. (C) Western blot of CD33 and GAPDH. (D) Levels of CD33 normalized to GAPDH. ***p < .001. All values represent the average and SEM of triplicate wells.