Table 1.
Statistics for Fig. 2, Gene mRNA levels differentially regulated and restored by low and high dose curcumin.
| A. CDllb, CDllc, iNOS, C1q, Arg1 (For Fig. 2A). | ||||
|---|---|---|---|---|
| MANOVA F(10,30) = 5.545 |
LSD post-hoc, LEVENE p < .05 |
|||
| Wilks = 0.123, n2 = 0.649, p < .00001 | Control vs Low | Control vs High | High vs Low | |
| logCDllb to control | F(2,19) = 4.087, p = .033 | 0.012 | 0.076 | NS |
| INOS normal control | F(2,19) = 6.950, p = .005 | 0.002 | 0.013 | NS |
| COX2 | F(2,18) =16.580, p = .0001 | 0.001 | 0.001 | NS |
| sqrtC1q to control | F(2,19) = 6.589, p = .007 | 0.050 | 0.112 | 0.002 |
| sqrtCD11c control | F(2,19) = 3.142, p = .066 | 0.021 | NS | 0.155 |
| ARG1 normal control | F(2,19) = 5.979, p = .01 | 0.107 | 0.003 | 0.148 |
| B. TMEM and P2yr12 (For Fig. 2A) | ||||
| MANOVA F(4,52) = 4.654 |
p Values for GAMES HOWELL post-hoc when LEVENE p > .05 |
|||
| Wilks = 0.542 n2 = 0.264 p < .003 | Control/Low | Control/High | High/Low | |
| TMEM normal Ctrl | F(2,27) = 8.057, p = .002 | 0.001 | NS | 0.085 |
| P2ry12 normal to control | F(2,27) = 7.222, p = .003 | 0.011 | NS | 0.008 |
| C. TREM2, CD33, CD68, TYROBP (For Fig. 2A) | ||||
| MANOVA F(8,48)=11.226 |
p Values for GAMES HOWELL post-hoc when Levene p > .05 |
|||
| Wilks = 0.12, n2 = 0.562, p < .0001 | Control/Low | Control/High | High/Low | |
| TREM2 | F(2,27) = 9.975, p = .001 | 0.0001 | NS | 0.002 |
| CD33 | F(2,27) = 10.735, p = .0001 | NS | 0.0001 | 0.095 |
| CD68 | F(2,27) = 9.788, p = .001 | 0.038 | NS | 0.055 |
| TYROBP | F(2,27) = 6.844, p = .004 | 0.049 | NS | 0.05 |
| D. TREM2 ratios (For Fig. 2B) | ||||
| MANOVA F(6,34) = 5.039 |
p values for LSD post-hoc, Levene p < .05 |
|||
| Wilks = 0.280, n2 = 0.471, p < .001 | Control vs Low | Control vs High | High vs Low | |
| TREM2/CD33 | F(2,19) = 19.11, p = .0001 | 0.0001 | 0.001 | 0.048 |
| LOG (TREM2/CD11b) | F(2,19) = 8.678, p = .002 | 0.001 | NS | 0.029 |
| log (TREM2/TMEM119) | F(2,19) = 5.247, p = .015 | 0.005 | NS | 0.044 |
| E. Log Trem-2 mRNA in hippocampus (For Fig. 2C) | ||||
| One Way ANOVA F(2,20) = 3.093, p = .070 |
p Values for LSD post-hoc, Levene p < .05 |
|||
| Control vs Low | Control vs High | High Vs Low | ||
| 0.028 | NS | NS | ||
| F. TREM2/CD33 buffy and hippocampus (hippo) (For Fig. 2D) | ||||
| UNIVARIATE | F(5,37) = 5.025 | p Values for Games Howell post-hoc, Levene p > .05 | ||
| 2-way dose (hi vs lo) × compartment (buffy vs hippo) | p = .013, r2 = 0.250 | Control vs Low | Control vs High | High Vs Low |
| Dose | F(2,37) = 6.538, p = .005 | buffy 0.001 | NS | 0.001 |
| Compartment (buffy vs hippo) | F(2,37) = 0.32, NS | hippo 0.050 | NS | NS |
| Dose* compartment | F(2,37) = 3.082, p = .06 | |||
| G. Log miRNA155 (for Fig. 2E) | ||||
| UNIVARIATE | F(3,28) = 16.482 | p Values for LSD post-hoc, Levene p = .354 | ||
| Two way Diet × FAD | p < .001 | FAD Curc vs FAD Control | Tg- Curc vs Tg-Control | FAD control vs Tg- control |
| Diet | F(1,28) = 18.528, p = .0001 | 0.001 | NS | p < .001 |
| FAD | F(1,28) = 11,225, p = .003 | |||
| Diet × FAD | F(1,28) = 7.624, p = .011 | |||