Figure 1.

Localization of the putative ligand(s) of hCD300f in vitro and in vivo. Primary cultures of the different glial central nervous system (CNS) cell types (A–E) or sections from mouse and rat brain (mouse brain, F–I) were used for co‐localization studies of hCD300f‐IgG2a (10 µg/mL) fusion protein and different cell‐specific markers. Small undifferentiated oligodendrocytes with few ramifications (A, GalC) and large highly ramified differentiated oligodendrocytes (B, GalC; C, myelin basic protein [MBP]) showed specific punctate staining with the fusion protein. Glial fibrillary acidic protein‐(GFAP) positive polygonal protoplasmic astrocytes did not show specific staining with the fusion proteins (D, arrow), being only positive the ramified fibrous GFAP‐stained cells. Microglial cells were negative for the staining with the fusion protein (E). A similar punctuate staining pattern was observed by confocal microscopy in the white matter of the brain stained with the fusion protein, which did not co‐localized with myelin markers (stained with CNPase, F, corpus callosum) but co‐localized with the oligodendrocyte soma and proximal projections (stained with APC, arrows in G–I). Scale bars: F–I 10 µm.