Skip to main content
. 2021 Apr 23;2021:5593655. doi: 10.1155/2021/5593655

Table 1.

Primer sequences and other information used to determine the expressions of pro- and anti-inflammatory cytokines.

Gene Primer sequence Amplicon size (bp) Annealing temperatures (°C)
IL-1α Forward (5′-3′)
GTGACTGCCCAAGATGAAGACC
Reverse (5′-3′)
TCCCAGAAGAAGAGGAGGTTGG
101 61

IL-6 Forward (5′-3′)
AGAGTAACATGTGTGAAAGCAGCA
Reverse (5′-3′)
TGATGATTTTCACCAGGCAAGTCT
129 61

IL-1β Forward (5′-3′)
AGGGACAGGATATGGAGCAACA
Reverse (5′-3′)
CTTTCAACACGCAGGACAGGT
129 61

IL-10 Forward (5′-3′)
TCAAGGCGCATGTGAACTCC
Reverse (5′-3′)
CATTCTTCACCTGCTCCACGG
120 61

TGF-β1 Forward (5′-3′)
CATGCCAACTTCTGCCTCGG
Reverse (5′-3′)
TGGTTGTACAGGGCCAGGAC
83 61

TNF-α Forward (5′-3′)
GACCTCTCTCTAATCAGCCCTCT
Reverse (5′-3′)
CTGGTTATCTCTCAGCTCCACG
174 61