Table 1.
Gene | Primer sequence | Amplicon size (bp) | Annealing temperatures (°C) |
---|---|---|---|
IL-1α | Forward (5′-3′) GTGACTGCCCAAGATGAAGACC Reverse (5′-3′) TCCCAGAAGAAGAGGAGGTTGG |
101 | 61 |
| |||
IL-6 | Forward (5′-3′) AGAGTAACATGTGTGAAAGCAGCA Reverse (5′-3′) TGATGATTTTCACCAGGCAAGTCT |
129 | 61 |
| |||
IL-1β | Forward (5′-3′) AGGGACAGGATATGGAGCAACA Reverse (5′-3′) CTTTCAACACGCAGGACAGGT |
129 | 61 |
| |||
IL-10 | Forward (5′-3′) TCAAGGCGCATGTGAACTCC Reverse (5′-3′) CATTCTTCACCTGCTCCACGG |
120 | 61 |
| |||
TGF-β1 | Forward (5′-3′) CATGCCAACTTCTGCCTCGG Reverse (5′-3′) TGGTTGTACAGGGCCAGGAC |
83 | 61 |
| |||
TNF-α | Forward (5′-3′) GACCTCTCTCTAATCAGCCCTCT Reverse (5′-3′) CTGGTTATCTCTCAGCTCCACG |
174 | 61 |