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Effects of camptothecin (CPT) on the AKT/NF-κB signaling pathway in DSS-induced ulcerative colitis in mice. Tissue lysates were prepared and subjected to western blot using (a, b) p-AKT, (a, c) p-IκBα, and (a, d) p-p65 antibodies. The p-AKT, p-p65, and p-IκBα immunoreactive bands were digitized and expressed as a ratio of AKT, IκBα, and p65 levels (b–d). Values are presented as mean ± SD (∗P < 0.05, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 vs. the LPS group).
