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. 2021 Apr 24;2021:5556659. doi: 10.1155/2021/5556659

Figure 8.

Figure 8

Effects of camptothecin (CPT) on LPS-induced activation of the MAPK signaling pathway in RAW264.7 cells. Total protein in RAW264.7 cells was collected after 4 h of LPS stimulation. CPT was added 1 h before LPS stimulation. Protein levels of (a, b) p-ERK1/2, (a, c) p-JNK1/2, and (a, d) p-P38 were detected by western blot and quantitatively assessed via densitometry using ERK1/2, JNK1/2, and P38 as internal controls. Protein levels were measured using ImageJ (http://imagej.nih.gov/ij/) and normalized to the protein levels of ERK1/2, JNK1/2, and P38. Values are presented as mean ± SD (∗∗P < 0.01 vs. LPS, ∗∗∗∗P < 0.0001 vs. LPS).