Table 2.

Primer sequences used to target TNF-α, IL-1β, IL-6, and β-actin.

Item	Primer	Amplicon length (bp)
TNF-α (sense)	5′-ACGGCATGGATCTCAAAGAC-3′	116
TNF-α (antisense)	5′-GTGGGTGAGGAGCACGTAGT-3′
IL-1β (sense)	5′-GCTGCTTCCAAACCTTTGAC-3′	121
IL-1β (antisense)	5′-AGCTTCTCCACAGCCACAAT-3′
IL-6 (sense)	5′-CCGGAGAGGAGACTTCACAG-3′	134
IL-6 (antisense)	5′-CAGAATTGCCATTGCACAAC-3′
iNOS (sense)	5′-GAACTGTAGCACAGCACAGGAAAT-3′	158
iNOS (antisense)	5′-CGTACCGGATGAGCTGTGAAT-3′
COX-2 (sense)	5′-CAGTTTATGTTGTCTGTCCAGAGTTTC-3′	127
COX-2 (antisense)	5′-CCAGCACTTCACCCATCAGTT-3′
β-Actin (sense)	5′-GTCAGGTCATCACTATCGGCAAT-3′	147
β-Actin (antisense)	5′-AGAGGTCTTTACGGATGTCAACGT-3′