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. 2021 Apr 24;2021:3043720. doi: 10.1155/2021/3043720

Figure 4.

Figure 4

Ethanol extract (EE) effect on Caenorhabditis elegans wild-type growth and development. Wild-type C. elegans animals were treated with EE at 0, 100, 250, 500, and 1000 μg/mL concentration. (a) Egg hatching assay. The x-axis corresponds to concentrations of EE. The y-axis corresponds to the percentage of egg hatching. (b) Body length was measured using Image J software. The x-axis corresponds to EE concentrations used. The y-axis corresponds to body length. (c) Image from the animal at 0 h and 48 h of EE treatment at the different concentrations. Three independent experiments were performed, with three replicates per treatment (A-B-C). The data were analysed by ANOVA and Tukey's test (P ≤ 0.05).