Fig. 1 |. Presymptomatic training improves motor performance on the rotarod in Rett mice.

a, Training regimen for naive, late-trained, and early-trained mice; each line represents 4 trials/day. b, Average motor performance on the rotarod. WT naive (n = 19), WT late-trained (n = 19), WT early-trained (n = 18), Rett naive (n = 19), Rett late-trained (n = 18), and Rett early-trained (n = 19) mice were tested across 4 days at 24 weeks of age. c, A subset of these mice underwent additional behavioral tests (see Extended Data Fig. 1) and the rest of the early-trained mice were followed for rotarod performance until 32 weeks of age. Early training delayed the onset of motor symptoms in Rett mice (n = 7) until after 22 weeks, with some benefit still apparent at 32 weeks; comparisons are WT (n = 7) at 32 weeks of age and naive Rett mice (n = 12) at 12 weeks of age. In another group of Rett mice (n = 8), we delayed training initiation until 16 weeks, following the same early-training protocol, but this yielded no benefit. The sample size (n) corresponds to the number of biologically independent mice. Data are represented as mean ± s.e.m. Statistical significance was determined using a two-way ANOVA with Tukey’s multiple comparisons test; ns (p>0.05), ** (p<0.01), *** (p<0.001), **** (p<0.0001).