Extended Data Fig. 1 |. Presymptomatic motor training on the rotarod does not improve other behavioral deficits in Rett mice.

a, Motor performance on the rotarod. WT (n = 12) and Rett (n = 10) mice were tested over 4 days beginning at 8 weeks of age. b, Timeline for additional behavioral assays in rotarod-trained mice. c, Performance curves of rotarod-trained Rett naive (n = 19), Rett late-trained (n = 18), and Rett early-trained (n = 19) mice across training and test days. d-o, After rotarod testing at 24 weeks of age, WT naive (n = 12), Rett naive (n = 12), WT late-trained (n = 12), Rett late-trained (n = 11), WT early-trained (n = 11), Rett early-trained (n = 12) mice were tested on a variety of behavioral assays. Motor function was tested in the footslip (d) and open field assays (e). Spatial memory was tested in the Morris water maze (f-h). Social behavior was tested in the 3-chamber assay (i). Anxiety was tested in the elevated plus maze (j). Sensorimotor gating was tested in the acoustic startle (k) and pre-pulse inhibition assays (l). Contextual (m) and cued (n) memory were assessed in the fear conditioning assays. Body weight was measured (o). The sample size (n) corresponds to the number of biologically independent mice. Data are represented as mean ± s.e.m. Statistical significance was determined using a two-way ANOVA with Tukey’s multiple comparisons test; ns (p>0.05), **** (p<0.0001).