Extended Data Fig. 2 |. Presymptomatic memory training in the Morris water maze does not improve other behavioral deficits in Rett mice.

a-c, Spatial memory performance in the Morris water maze. WT (n = 8) and Rett (n = 8) mice were tested over 4 days beginning at 4 weeks of age (a). A probe trial on day 5 revealed the time spent in the target quadrant (b) and the number of platform area crossings (c). d, Performance curves of Morris water maze-trained Rett naive (n = 19), Rett late-trained (n = 19), and Rett early-trained (n = 18) mice across training and test days. e, Spatial memory in the Morris water maze over time in early-trained Rett mice (n = 7) and Rett mice (n = 8) that missed a training session. f, Swim speed in the Morris water maze of WT naive (n = 18), WT late-trained (n = 18), WT early-trained (n = 19), Rett naive (n = 19), Rett late-trained (n = 19), and Rett early-trained (n = 18) mice. g, Timeline for additional behavioral assays in Morris water maze-trained mice. h-o, After Morris water maze testing at 12 weeks of age, WT naive (n = 11), Rett naive (n = 12), WT late-trained (n = 11), Rett late-trained (n = 12), WT early-trained (n = 12), Rett early-trained (n = 11) mice were tested on a variety of behavioral assays. Motor function was tested in rotarod (h), footslip (i), and open field assays (j). Body weight was measured (k). Social behavior was tested in the 3-chamber assay (l). Anxiety was tested in the elevated plus maze (m). Sensorimotor gating was tested in the acoustic startle (n) and pre-pulse inhibition (o) assays. The sample size (n) corresponds to the number of biologically independent mice. Data are represented as mean ± s.e.m. Statistical significance was determined using a two-tailed, unpaired student’s t-test (b-c) or two-way ANOVA with Tukey’s multiple comparisons test (a, d-o); ns (p>0.05), **** (p<0.0001).