Fig. 1.

Leukemia progression was delayed with haplo-SCT in AML-ETO leukemic mice. a Schematic illustration shows the establishment of nonirradiated leukemia mouse models which carried human AML-ETO fusion gene of haplo-identical and major histocompatibility (MHC)-matched transplantation models. b Kaplan–Meier survival curves compare survival between MHC-matched and haplo-SCT AML-ETO mice. The median survival was 13.5 days in the MHC-matched group and 21 days in the haplo group. Mantel-Cox test; n = 10. Heat map of cluster frequency from peripheral blood (c) or spleen (d) including leukemia cells (GFP⁺), CD3⁺CD4⁺ T cells, CD3⁺CD8⁺ T cells and CD3^-CD49b⁺ NK cells. There are 6 animals (for peripheral blood) or 3 animals (for spleen) in each group per timepoint. The white part represents there is no lived mice in the MHC-matched group at that timepoint. e Representative multispectral immunofluorescence images of FFPE spleen sections in different tumor burden (T1 and T2) or timepoint after transplantation. f Quantified immune cells (proportion of all cells) of MHC-matched or haplo group in different tumor burden. n = 3. Error bars represent the mean ± SEM, ***P < 0.001, **P < 0.01, *P < 0.05