Fig. 2.

Elevated CTL levels in haplo group increased leukemia cell apoptosis compared with MHC-matched group. a Comparison of the percentages of AML cells, T cells and NK cells in the spleen of MHC-matched, haplo and haplo-late group of AML-ETO leukemic mice. b Comparison of the percentages of CD4⁺ T cells and CD8⁺ T cells in the splenic CD3⁺ T cell subsets of MHC-matched, haplo and haplo-late group of AML-ETO leukemic mice. c Representative flow cytometry results show apoptotic CD4⁺ T cells from the spleens of the MHC-matched, haplo and haplo-late groups. d Comparison of the percentages of apoptotic immune cells from spleen including CD4⁺ T cell, CD8⁺ T cells and NK cells in MHC-matched, haplo and haplo-late group. e Representative flow cytometry results show the TNF-α secretion by CD4⁺ T cells from spleen in MHC-matched, haplo and haplo-late group. Cytotoxic cytokine secretion of CD4⁺ T cells (f) and CD8⁺ T cells (g) from spleen in MHC-matched, haplo and haplo-late group. h Representative flow cytometry results show the Perforin and CD107a secretion by NK cells from spleen in MHC-matched, haplo and haplo-late group. i Cytotoxic cytokine secretion of NK cells from spleen in MHC-matched, haplo and haplo-late group. Representative flow cytometry results show apoptotic leukemia cells (j) and percentages of apoptotic leukemia cells (k) from the spleens or the BM of the MHC-matched, haplo and haplo-late groups. Error bars represent the mean ± SEM, ***P < 0.001, **P < 0.01, *P < 0.05