Figure 12.

Scheme illustrating covalent labeling of Asp and Glu residues by EDC/sulfo-NHS activation and the subsequent biotinyl cystamine reaction. After cell lysis and protein digestion, peptides from membrane proteins are enriched via the binding between biotinylated Asp/Glu and neutravidin column. The addition of dithiothreitol (DTT) was applied to elute the peptides followed by iodoacetamide (IAM) alkylation. Figure used with permission from Müller et al., 2019, DOI: 10.1038/s41598-019-52188-4.