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Generation of Fgf9 knockout allele. (A) Generations of Fgf9 allele lacking exon 2. Purple boxes, exons. Gray boxes, introns. Orange triangles, loxP sites. pGK-NEO, pGK-NEO cassette for positive selection. TK, thymidine kinase cassette included for negative selection. PCR-based genotyping of (B) Fgf9F/+ (F indicates a floxed allele), (C) Ddx4–Cre, (D) Fgf9+/–, Wildtype, and Fgf9–/– mice.
