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. 2021 Apr 20;12:653040. doi: 10.3389/fphys.2021.653040

FIGURE 3.

FIGURE 3

Aberrant morphology and fusion failure in Fgf9–/– embryos at E18.5. The HE staining of (A–A”) anterior, (C–C”) middle, and (E–E”) posterior palate in Wildtype, Fgf9+/– and Fgf9–/– embryos The magnified view showing MASSON staining of (B–B”) anterior, (D–D”) middle, and (F–F”) posterior palate in Wildtype, Fgf9+/–, and Fgf9–/– embryos. Black arrows indicate the premature trabecular bone development. Red arrows indicate the thickened cuticular layer. *, cleft secondary palate. P, palate. MAX, maxilla. MAN, mandible. T, tongue. TB, trabecular bone. M, molar. Scale bars = 200 μm.