Table 2.
Forty primer pairs developed from the ‘Golden Delicious’ doubled haploid genome in the region from 31,092,690 to 32,362,417.
| Primer name | Forward sequence | Reverse sequence |
|---|---|---|
| FRMb31M22 | TGGATTGAAAATTTGGGTGTC | GCTGGTGCATTTCCATTTTT |
| FRMb31M23 | GACACCAAAACGAGCCCTTA | TCCATTTTTGGATTCTGAAGTTG |
| FRMb31M27a | AATGTGTGGTTCCTCCCAAA | CACGAGTTAACATTACCTTTGATTG |
| FRMb31M27b | TCTGAAGAGGAGATCAGAGAACC | GCCCAATTTCGGTTACACAT |
| FRMb31M30 | CCAACTTTAAGGATCCAAATCG | CACAGCAGGTCGGACATTT |
| FRMb31M31 | AACAGGTGCTTGAATAGTTGACA | CCAGCAATTACTGAAAGAGATCA |
| FRMb31M31a | AGCGCGTGGGACTATAGAAA | GGCTTTCTGAATTGCTTTGC |
| FRMb31M31b | GAGTGTTCAGAGCCCAAAGC | TTGGTGAAATGGGTGTCAGA |
| FRMb31M33 | GTGGTTCCGGAATTGAGAGA | AGAATCAGAAGCCCTTCACG |
| FRMb31M35 | GCCATTGTGATGATCTGATTTT | GCTGAGGGATGAGTTTTTGC |
| FRMb31M36a | ATGCATTTCATGGTTGCATA | TCACAATCACCCTAGATGCAG |
| FRMb31M36b | TCTTGGCATGTCATACATTTGAG | AAACGTGAGTGGGCAAAAAC |
| FRMb31M48 | TTGCCTCTTACCCCCTTACC | AGATCGGGGAGAAGGAGAAA |
| FRMb31M52 | AATTCCCAAGTTGCTGCTTC | CGGAATTCTGTTATTGCATTTTT |
| FRMb31M54 | CCCTAACCAATCGAATCGAAA | CTCCCGGAATTCATCAAGAC |
| FRMb31M57 | TCAAGACCAAGGCTTCACCT | GGCCCAAAGAAGAGAAGGTC |
| FRMb31M61a | GGTGGGGTTGAAAGATGAGA | AAGCAGCATCACTGGTGAGA |
| FRMb31M61b | GGGAGGAGGTTTGTCCCTTA | TGATGATGGTCCTTGTTGGA |
| FRMb31M67 | CGCCTTGCCTAGACTCGTAT | CCGACTTAATCGTCAGCACA |
| FRMb31M69 | AAGGAGCTAGCTTGCACTGG | GAGAGGGAAGGGATTTCACA |
| FRMb31M7 | CAGACTCTGCAACCCCTCTC | AGTCAAGTGCTGCTGCAAGA |
| FRMb31M80 | CTGGCCGTAGATGATCCAGT | GTTTGGCGAGAGAGAAAACG |
| FRMb31M82 | TAGGCTCTGGTGCCAAGAAG | CAGATCACGGAACCCTAGAGA |
| FRMb31M84a | GGGCCAATGTCACACTAACA | ACGAACACGACAGACACGAC |
| FRMb31M84b | TGCACATCCATATTCCATCA | GTGGGTAACACGAAACACGA |
| FRMb31M85 | CTGGCCGTAGATGATCCAGT | GTTTGGCGAGAGAGAAAACG |
| FRMb31M87 | AAAGAGCTTTGCTTGGCTTG | TCTCAACTTTCCCACCAACC |
| FRMb32M039 | CTCAGCCTGCTAGAGGAGCTA | CAGGCAAGTCTGATATTCTTGG |
| FRMb32M03x_F1* | TCTCAGCTTTTGAAGCACCA | CACCTTTGTTCCATCCGTCT |
| FRMb32M03x_F2* | TGAACGCCGATAGAGATTGA | |
| FRMb32M04a | GCTTTGGATTCCAGTTTAGATAAG | TTTGCTTGTATTTTCCATGACTG |
| FRMb32M04b | TGGACAAATTCAGTGACACCA | CAAACCACCCCAAATTCTGT |
| FRMb32M11 | AAACATGAACCGGTTTGTCC | GCTGGCGGATGGATAGATTA |
| FRMb32M12 | TGTCAATGATTTGTCCCCATA | TTGCAGCAAGGCTCATAAAA |
| FRMb32M16 | GAGCCCCATGAACCTCAGTA | CCTGGCCATGCAATCTATCT |
| FRMb32M19 | GTCGGCCATTCCTAAAACAA | AGAGTGGTGCATCATTGCAG |
| FRMb32M21 | TTCTTGCATAATTTGCTCTGTGA | CAGTGGAGGAAAGGCAACAT |
| FRMb32M27 | TTTAATTGGCTTTTCATTCACG | AAGGCGACTCATGATTTCGTA |
| FRMb32M33x | CCTTTGTTGGAAGGAGCTACA | CCATCTTACTCATTGTCGCTTA |
| FRMb32M33y | ATGCCCATCATCTTCTCCAC | TGGTAGAGCGTGGTATGGAA |
*
Both forward primers had the same reverse primer.