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. 2021 Apr 20;12:645210. doi: 10.3389/fimmu.2021.645210

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Copyright © 2021 Rosenbaum, Tchitchek, Joly, Rodriguez Pozo, Stimmer, Langlois, Hocini, Gosse, Pejoski, Cosma, Beignon, Dereuddre-Bosquet, Levy, Le Grand and Martinon

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of the SC/ID/IM routes on early systemic immunity. (A) Heatmap representation showing the changes in cell abundance (in log2 fold change) of populations characterized by flow cytometry in the blood 3, 6, and 24 h after SC/ID/IM MVA injection. Populations were organized based on their cell abundance by hierarchical clustering and are represented by a dendrogram. Changes in cell abundance of each population were normalized and are represented by a color-gradient scale ranging from yellow to red. Significant changes over time are indicated on the right (B) Scatter plot showing the kinetics of changes in cell abundance for selected cell populations. Each dot represents one single biological sample. ns, non-significant; *0.05 > p > 0.01; **0.01 > p > 0.001; ***0.001 > p > 0.0001; ****p < 0.0001.