Figure 2.

Knockdown of FYCO1 Inhibits Cardiomyocyte Autophagy

(A) FYCO1 mRNA and protein expression in NRCMs after adenoviral transduction (MOI = 25) with 2 different synthetic micro–ribonucleic acids (microRNAs) that target FYCO1. Data are presented as dot plots with mean ± SEM (2-sided Student’s t-test). (B) LC3-II expression in NRCMs transduced with synthetic microRNA against FYCO1 and treated with glucose deprivation for 24 h. Data are presented as dot plots with mean ± SEM (2-way ANOVA followed by Student-Newman-Keuls post hoc tests). (C) Quantification of autophagosomes labeled by GFP-LC3 in NRCMs that overexpress a microRNA against FYCO1 and have been starved by glucose deprivation for 24 h. Data are presented as box plots with median and 5th and 95th percentile whiskers (Kruskal-Wallis ANOVA on ranks followed by Wilcoxon rank sum tests). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗ p < 0.001. Scale bar, 10 μm. Abbreviations as in Figure 1.