Figure 4.

Fyco1-Deficient Mice Are Resistant to Autophagy Induced by Pressure Overload

Fyco1-KO mice and WT littermates were subjected to transverse aortic constriction (TAC). Ratio of left ventricular (LV) mass to body weight (A) and echocardiographic analyses of LV end-diastolic diameter (LVEDD) (B) and fractional shortening (C) after 1 week of TAC. (D) Quantification of Nppa/ANF (atrial natriuretic factor) and Nppb/BNP (brain natriuretic peptide) expression after 1 week of TAC. (E) LV mass/body weight and echocardiographic analyses of (F) LVEDD and (G) fractional shortening after 2 weeks of TAC. (H) Lung weight after 2 weeks of TAC. LC3-II protein expression in the left ventricle after 1 week (I) and after 2 weeks (J) of severe TAC. All datasets are presented as dot plots with mean ± SEM (2-way ANOVA followed by Student-Newman-Keuls post hoc tests). Kaplan-Meier survival curves of WT and KO mice during (K) 1 week and during (L) 2 weeks of TAC. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. Abbreviations as in Figure 1.