Figure 5.

FYCO1-TG Mice Reveal Mild Cardiac Hypertrophy Without Induction of Maladaptive Hypertrophic Genes

(A) Heart/body weight ratios of transgenic (TG) mice with cardiac-specific overexpression of FYCO1 (TG) and their WT littermates. (B) Echocardiographic analyses of interventricular septal thickness in diastole (IVSd), left ventricular posterior wall thickness in diastole (LVPWd), and fractional shortening. (C) Lung weight of TG and WT mice. Data are presented as dot plots with mean ± SEM (2-sided Student’s t test). (D) Quantification of cardiomyocyte CSAs by staining of TG and WT heart sections with wheat germ agglutinin lectin. Data are presented as box plots with median and 5th and 95th percentile whiskers (2-tailed Mann-Whitney rank sum test). (E) Gene expression of Nppa, Nppb, and Rcan1.4 by quantitative polymerase chain reaction (qPCR). Data are presented as dot plots with mean ± SEM (2-sided Student’s t-test). (F) Quantification of CSA in isolated NRCMs that overexpress FYCO1. Data are presented as box plots with median and 5th and 95th percentile whiskers (2-tailed Mann-Whitney rank sum test). (G) Expression of hypertrophic genes Nppa, Nppb, and Rcan1.4 by qPCR in NRCMs after overexpression of FYCO1 (MOI = 50). Data are presented as dot plots with mean ± SEM (2-sided Student’s t-test). ∗p < 0.05 and ∗∗∗p < 0.001. Abbreviations as in Figure 1.